Figure 9.
Acute activation of Sim1PVH neurons increases subcutaneous intrascapular temperature. Sim1-Cre+3Dq, OXT-iCre+3Dq, and WT+3Dq mice received temperature transponders in the subcutaneous intrascapular tissue directly above BAT. TIS was measured before and after vehicle or CNO administration. A, B, TIS is shown relative to baseline TIS before and after injection of vehicle or CNO (A, dashed and solid lines, respectively) and also is represented as the peak area under the curve (B). Average values ± SEM are shown. *p < 0.05, **p < 0.01, ***p < 0.001 compared with vehicle values (WT, n = 5; Sim1-Cre, n = 3; OXT-iCre, n = 4). Significance was determined using repeated-measures two-way ANOVA with Sidak multiple-comparisons post hoc test.