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. 2014 Oct 24;11:51. doi: 10.1186/1743-7075-11-51

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© Corware et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Impact of malnutrition on parasite replication and arginase activity in Leishmania infantum -infected BALB/c mice. Groups of BALB/c (n =5) mice were fed with a low protein diet (=malnourished (MN) group, 0.7% protein) or normal diet (=control group, 14.4% protein) and 7 days later, were infected with L. infantum. After nine days, parasite load (A) and arginase activity were measured in the spleens (B). BMMΦ were activated with IL-4, IFN-γ and TNF-α or left unactivated (nil) and were infected with L. infantum for 4 days and parasite replication (C) and arginase activity (D) were determined as described in material and methods. Data show the results of one representative experiment out of two independent experiments. *: p <0.05. nd = not detectable.