Table 1.
Characteristics of specimens sent for BCG identification, clinical MTB isolates and reference strains
|
Samples |
Disease description and management |
Specimen site |
PCR |
result |
PCR |
oxyR *
|
SD TB Ag MPT64 Rapid test |
AccuProbe Complex test |
|---|---|---|---|---|---|---|---|---|
| RD1 | RD9 | hsp65 | ||||||
|
For BCG identification (
n
=16) | ||||||||
| Case/Sex/Age (months) | ||||||||
| 1/M/3 |
BCG adentitis. Excision of inguinal lymph node |
Inguinal lymph node |
― |
― |
+ |
M. bovis |
NEG |
ND |
| 2/M/2 |
Incision and drainage of abscess |
Axillary abscess |
― |
― |
+ |
M. bovis |
NEG |
POS |
| 3/M/2 |
No information available |
Axillary abscess |
― |
― |
+ |
M. bovis |
NEG |
POS |
| 4/F/4 |
Incision and drainage of gluteal abscess |
Injection site abscess |
― |
― |
+ |
M. bovis |
NEG |
POS |
| 5/M/3 |
BCG adentitis. Excision of inguinal lymph node |
Inguinal lymph node |
― |
― |
+ |
M. bovis |
NEG |
POS |
| 6/F/3 |
No information available |
Inguinal lymph node |
― |
― |
+ |
M. bovis |
NEG |
POS |
| 7/M/3 |
No information available |
Inguinal lymph node |
― |
― |
+ |
M. bovis |
NEG |
POS |
| 8/F/3 |
No information available |
Inguinal lymph node |
― |
― |
+ |
M. bovis |
NEG |
POS |
| 9/M/4 |
BCG adenitis. Excision of inguinal lymph node |
Injection site abscess |
― |
― |
+ |
M. bovis |
NEG |
POS |
| 10/M/22 |
No information available |
Lymph node |
― |
― |
+ |
M. bovis |
NEG |
POS |
| 11/M/2 |
No information available |
Lymph node |
― |
― |
+ |
M. bovis |
NEG |
POS |
| 12/F/3 |
Left caseating inguinal lymph node. |
Lymph node |
― |
― |
+ |
M. bovis |
NEG |
POS |
| 13/M/3 |
No information available |
Inguinal lymph node |
― |
― |
+ |
M. bovis |
NEG |
POS |
| 14/M/4 |
Left axillary lymph node abscess. Incision and drainage |
Axillary abscess |
― |
― |
+ |
M. bovis |
NEG |
POS |
| 15/M/3 |
No information available |
Lymph node abscess |
― |
― |
+ |
M. bovis |
NEG |
POS |
| 16/M/3 |
No information available |
Inguinal lymph node aspirate |
― |
― |
+ |
M. bovis |
NEG |
POS |
|
For specificity and sensitivity testing | ||||||||
| Clinical MTB isolates (n= 32) |
|
Respiratory and non-respiratory |
+ |
+ |
+ |
ND |
POS |
POS |
| M. tuberculosis complex control strains (n=3) |
|
|
|
|
|
|
|
|
| M. bovis BCG Pasteur ATCC 35734 |
NA |
― |
― |
+ |
M. bovis |
NEG |
POS |
|
| M. tuberculosis H37Ra ATCC 25177 |
NA |
+ |
+ |
+ |
MTB |
POS |
POS |
|
| M. tuberculosis H37Rv ATCC 27294 |
NA |
+ |
+ |
+ |
MTB |
POS |
POS |
|
| Non M. tuberculosis strains (n=13) |
|
|
|
|
|
|
|
|
| M. intracellulare ATCC 13950 |
NA |
― |
― |
― |
ND |
ND- |
ND |
|
| M. gordonae ATCC 35756 |
NA |
― |
― |
― |
ND |
ND |
ND |
|
| M. kansasii ATCC12478 |
NA |
― |
― |
― |
ND |
ND |
ND |
|
| M. septicum ATCC 700731 |
NA |
― |
― |
― |
ND |
ND |
ND |
|
| M. senegalense ATCC 35755 |
NA |
― |
― |
― |
ND |
ND |
ND |
|
| M. perigrinum ATCC 23001 |
NA |
― |
― |
― |
ND |
ND |
ND |
|
| M. xenopi ATCC 19250 |
NA |
― |
― |
― |
ND |
ND |
ND |
|
| M. abscessus ATCC 19977 |
NA |
― |
― |
― |
ND |
ND |
ND |
|
| M. chelonae ATCC 19539 |
NA |
― |
― |
― |
ND |
ND |
ND |
|
| M. fortuitum ATCC 6841 |
NA |
― |
― |
― |
ND |
ND |
ND |
|
| M. haemophilum ATCC 29548 |
NA |
― |
― |
― |
ND |
ND |
ND |
|
| Rhodococcus equi |
NA |
― |
― |
― |
ND |
ND |
ND |
|
| Norcardia farcinica | NA | ― | ― | ― | ND | ND | ND | |
ND: Not done.
NA: Not applicable.
— : No PCR amplification observed.
+ : Positive PCR amplification.
oxyR: A single nucleotide polymorphism, G➔A at position 285, revealed by oxyR sequencing differentiates M. bovis and TBC.