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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: Gastroenterology. 2013 Nov 1;146(2):539–49.e7. doi: 10.1053/j.gastro.2013.10.059

Fig. 2. Production and secretion of hepatic FGF21 are reduced in SIRT1 LKO mice in response to nutrient deprivation.

Fig. 2

SIRT1 LKO mice and their WT littermates at 6- and 7-months of age (n = 4-7) were subjected to feeding (Fed), fasting for 24 h (Fasted), or refeeding for 6 h following a 24-h fast (Refed). A. Hepatic SIRT1 expression is increased by fasting and decreased by refeeding in WT mice, and the induction is eliminated in SIRT1 LKO mice (n = 4-7). B and C. Fasting-induced gene expression and protein production of hepatic FGF21 are diminished in SIRT1 LKO mice (n = 4-7), as determined by real-time PCR and ELISA assays. D. The circulating levels of FGF21 are robustly elevated by fasting in WT mice, and the induction is impaired in SIRT1 LKO mice. *P<0.05, vs. the fed WT mice; #P<0.05, vs. the fasted WT mice.