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Identification of CDPK3 expression in E. coli BL21 (DE3) by SDS-PAGE and western-blotting. M: protein molecular weight marker; lane 1, recombinant pET-30a (+); lane 2, rTgCDPK3 induced for 8 h with 0.6 mmol/L IPTG; lane 3, Elution result of 20 mmol/L imidazole solution; lane 4, Elution result of 50 mmol/L imidazole solution; lane 5, Western blotting analysis with the sera against pVAX-CDPK3; lane 6, Western blotting analysis with the sera before immunization.
