Table 6.
Analyte | Conc. added (ng/ml) | Short-term stability |
Autosampler stability |
Freeze-thaw stability (three cycles) |
Four-week stability |
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Conc. found (%) | RSD (%) | Conc. found (%) | RSD (%) | Conc. found (%) | RSD (%) | Conc. found (%) | RSD (%) | ||
GC | 2.5 | 98.42±5.67 | 5.76 | 95.28±9.00 | 9.45 | 97.15±10.03 | 10.32 | 98.10±7.21 | 7.35 |
10 | 101.02±4.35 | 4.31 | 104.95±2.84 | 2.71 | 102.94±3.48 | 3.38 | 103.76±8.29 | 7.99 | |
50 | 103.40±9.78 | 9.46 | 97.40±5.16 | 5.29 | 98.37±4.81 | 4.89 | 97.57±3.42 | 3.51 | |
BB | 12.5 | 87.60±4.52 | 5.16 | 84.59±10.79 | 12.76 | 90.10±5.27 | 5.85 | 105.67±10.12 | 9.58 |
50 | 97.56±4.66 | 4.78 | 122.30±2.19 | 1.79 | 109.64±5.00 | 4.56 | 96.74±9.01 | 9.31 | |
250 | 105.12±10.24 | 9.74 | 100.84±5.90 | 5.85 | 102.29±5.30 | 5.18 | 97.11±10.08 | 10.38 | |
QCT | 2.5 | 101.65±3.41 | 3.35 | 104.43±7.73 | 7.40 | 98.72±6.81 | 6.90 | 101.31±11.09 | 10.95 |
10 | 104.42±4.56 | 4.37 | 113.21±7.98 | 7.05 | 101.21±8.75 | 8.65 | 97.10±7.23 | 7.45 | |
50 | 101.74±7.88 | 7.75 | 98.97±9.05 | 9.14 | 105.31±7.64 | 7.25 | 102.35±8.22 | 8.03 | |
GB | 6.25 | 93.72±5.67 | 6.05 | 105.71±3.57 | 3.37 | 105.20±9.27 | 8.81 | 98.70±8.34 | 8.45 |
25 | 98.67±6.78 | 6.87 | 114.43±1.55 | 1.35 | 104.52±5.65 | 5.41 | 100.78±5.48 | 5.44 | |
125 | 104.14±7.99 | 7.67 | 108.76±5.10 | 4.69 | 110.19±3.39 | 3.07 | 95.77±5.63 | 5.88 | |
GA | 12.5 | 106.24±10.34 | 9.73 | 79.73±5.88 | 7.37 | 89.05±4.91 | 5.51 | 97.70±6.80 | 6.96 |
50 | 109.12±4.01 | 3.67 | 118.25±3.20 | 2.71 | 103.68±6.47 | 6.24 | 101.21±5.84 | 5.77 | |
250 | 90.31±6.74 | 7.46 | 89.14±3.13 | 3.52 | 90.97±2.98 | 3.28 | 98.81±4.45 | 4.50 | |
KMF | 2.5 | 107.34±5.43 | 5.06 | 96.83±9.89 | 10.21 | 96.55±7.32 | 7.58 | 97.99±7.22 | 7.37 |
10 | 100.72±2.45 | 2.43 | 98.73±4.82 | 4.88 | 104.93±7.54 | 7.19 | 102.31±6.78 | 6.63 | |
50 | 98.97±3.21 | 3.24 | 102.3±7.15 | 6.99 | 99.74±8.71 | 8.73 | 108.98±12.11 | 11.11 | |
ISR | 2.5 | 104.54±4.54 | 4.34 | 87.98±5.75 | 6.54 | 89.74±5.25 | 5.85 | 101.16±9.12 | 9.02 |
10 | 101.23±6.77 | 6.69 | 112.32±5.78 | 5.15 | 101.72±5.67 | 5.57 | 103.45±7.76 | 7.50 | |
50 | 98.78±7.68 | 7.77 | 100.44±6.91 | 6.88 | 105.50±9.78 | 9.27 | 98.79±6.34 | 6.42 |
The values for the concentration found are expressed as mean±SD, n=3. GC: ginkgolide C; BB: bilobalide; QCT: quercetin; GB: ginkgolide B; GA: ginkgolide A; KMF: kaempferol; ISR: isorhamnetin; RSD: relative standard deviation