Table 1.
Lack of C-C chemokine receptor 2 (CCR2−/−) and interventions affect body and tissue masses, tibial length, and serum markers
| wildtype | CCR2−/− | Two-way ANOVA P-values | |||||||
|---|---|---|---|---|---|---|---|---|---|
| Control (n=10) |
OVX (n=10) |
DEN (n=10) |
Control (n=9) |
OVX (n=8) |
DEN (n=10) |
Genotype | Intervention | Interaction | |
| Body mass: | |||||||||
| Pre-intervention mass (g) | 20.99 (0.47) | 21.17 (0.47) | 20.67 (0.47) | 23.17 (0.50) | 23.40 (0.53) | 22.18 (0.47) | <0.001 | 0.183 | 0.704 |
| Mass at sacrifice (g) | 23.34 (0.88) | 27.57 (0.88) | 23.02 (0.88) | 27.34 (0.93) | 32.59 (0.99) | 26.63 (0.88) | <0.001 | <0.001 | 0.733 |
| Tibial bone mass and length: | |||||||||
| Wet mass (mg) | 43.64 (0.80) | 43.26 (0.76) | 40.69 (0.76) | 46.08 (0.80) | 45.75 (0.85) | 43.34 (0.88) | <0.001 | <0.001 | 0.990 |
| Dry mass (mg) | 26.35 (0.45) | 26.19 (0.43) | 23.74 (0.43) | 28.46 (0.45) | 27.93 (0.48) | 26.97 (0.43) | <0.001 | <0.001 | 0.850 |
| Length (mm) | 17.91 (0.08) | 18.37 (0.08) | 18.04 (0.08) | 18.43 (0.08) | 18.48 (0.09) | 18.33 (0.08) | <0.001 | 0.005 | 0.051 |
| Markers of bone turnover and collagen content: | |||||||||
| Osteocalcin (ng/mL) | 25.24 (2.0) | 30.72 (2.0) | 30.94 (2.0) | 29.64 (2.1) | 28.47 (2.2) | 27.86 (2.0) | 0.853 | 0.506 | 0.137 |
| CTX (ng/mL) | 15.76 (1.41) | 18.90 (1.41) | 16.94 (1.49) | 15.43 (1.49) | 17.59 (1.58) | 18.60 (1.41) | 0.165 | 0.996 | 0.590 |
| Hydroxyproline content (µg) | 35.65 (0.71) | 35.32 (0.68) | 35.83 (0.68) | 36.29 (0.71) | 36.52 (0.76) | 34.72 (0.68) | 0.672 | 0.535 | 0.227 |
| Mechanical properties: | |||||||||
| Failure load (N) | 6.59 (0.61) | 5.61 (0.64) | 5.68 (0.61) | 7.63 (0.65) | 5.84 (0.68) | 7.62 (0.61) | 0.045 | 0.105 | 0.412 |
| Post yield displacement (mm) | 0.437 (0.084) | 0.475 (0.089) | 0.383 (0.084) | 0.326 (0.089) | 0.452 (0.094) | 0.228 (0.084) | 0.184 | 0.209 | 0.750 |
Values are given as mean (SEM)