Skip to main content
. Author manuscript; available in PMC: 2015 May 10.
Published in final edited form as: Nat Commun. 2014 Nov 10;5:5416. doi: 10.1038/ncomms6416

Figure 3. S6K1 activation and SOCS3 deletion act additively to promote axon regeneration.

Figure 3

(a) Confocal images of flat-mounted retinas showing surviving Tuj1 positive RGCs and pS6 immunostaining, 2 weeks after ON crush in SOCS3f/f mice injected with either AAV-Cre or AAV-Cre +AAV-S6K1-CA. Scale bar, 20 μm. (b) Quantification of surviving RGCs, represented as percentage of TUJ1 positive RGCs in the injured eye, compared to the intact contralateral eye, 2 weeks after ON crush. Data are presented as means ± s.e.m. (c) Confocal images of ON longitudinal sections showing regenerating fibers labeled with CTB 2 weeks after ON crush. *: crush site. Scale bar, 100 μm. (d) Quantification of regenerating fibers at different distances distal to the lesion site. *: p<0.05. Data are presented as means ± s.e.m, n=8–12.