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. Author manuscript; available in PMC: 2015 Aug 1.
Published in final edited form as: Free Radic Biol Med. 2014 May 14;73:201–213. doi: 10.1016/j.freeradbiomed.2014.04.029

Figure 2. PH is associated with increased vascular ROS production and Nox2 and Nox4 expression.

Figure 2

(A) Localization of in vivo reactive oxygen species (ROS) in pulmonary arterioles. Sections of control and 4wk MCT-treated rat lungs were co-stained for fibroblast activation protein (FAP), 8 Hydroxy-2′dexoyguanosine (ROS marker) and DAPI. (B) ROS production was quantified by an Oxidative DNA Damage ELISA kit using genomic DNA isolated from control and 4wk MCT-treated rat lungs. Nox2 and Nox4 protein (C) and mRNA (D) expression were measured in isolated PA from MCT rats. Results are representative of at least 3–5 separate experiments. * different from Vehicle, p < 0.05 (n = 5–6).