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. Author manuscript; available in PMC: 2015 Aug 1.
Published in final edited form as: Free Radic Biol Med. 2014 May 14;73:201–213. doi: 10.1016/j.freeradbiomed.2014.04.029

Figure 5. Cav-1 alters the expression of Nox proteins in native cells.

Figure 5

(A) Macrophages were transduced with either Cav-1 adenovirus (30MOI) or a non-specific control gene (RFP) (30MOI) for 48hrs, and cell lysates were immunoblotted for Nox2, Cav-1, and GAPDH. (B) Human lung fibroblasts were exposed to increasing amounts of Cav-1 adenovirus (10 and 30MOI) and cell lysates immunoblotted for Nox4, Cav-1, and GAPDH. (C) Macrophages from WT or Cav-1 knockout mice were isolated, and cell lysates immunoblotted for Nox2, Cav-1, and GAPDH. (D) Cav-1 expression was silenced using siRNA (10(+), 30(++) nM) in human lung fibroblasts, and cell lysates immunoblotted for Nox4, Cav-1, and GAPDH. Results are representative of at least 3–5 separate experiments.