Figure 7. Cav-1 knockout mice exhibit increased Nox2 and Nox4 mRNA and protein expression and increased pulmonary hypertension.
(A) Protein expression of Nox2 and Nox4 in whole lung lysates from WT or Cav-1−/− mice exposed to hypoxia was determined by Western blot. (B) Nox2 and Nox4 mRNA expression were determined by qRT-PCR (ΔΔCt) normalized to GAPDH in lung tissue from WT or Cav-1−/− mice exposed to hypoxia. *different from wild type, p < 0.05 (n = 3). (C) Hypoxia-induced PH is enhanced in Cav1−/− mice, * versus normoxia, + versus WT p<0.05 (n=3–5).