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. 2014 Nov 12;5:431. doi: 10.3389/fphys.2014.00431

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Copyright © 2014 Sitdikova, Fuchs, Kainz, Weiger and Hermann.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Mean values of BK channel open time probabilities (P_open). Three types of channels, GH3-BK (A), GH4-BK (B), and GH4-STREX (C) cells, were investigated. Bar graphs show Po-values under control conditions (left bars, white) and after extracellular application of NaHS (right bars, gray). ATP, kinase, kinase inhibitor, kinase inhibitor staurosporine (STS), or phosphatase blocker okadaic acid (OA) were added to the pipette solution; ATP0 – no ATP added; ATP1 – 1 mM ATP added; PKA (50 units); PKCsu (0.1 units/ml); PKCin – 500 nM; STS – 1 μM; OA – 100 nM. Figures are based on back transformed means and s.e.m. values (error bars), respectively. Asteriks indicate significance of H₂S (300 μM NaHS equivalent to effective 34–41 μM H₂S) effects according to the logit transformed data.