Table 2.
Phosphorylation of BK channels.
| Popen | |||
|---|---|---|---|
| GH3 | GH4 | GH4 STREX | |
| ATP 0 n = 12 | 0.0152 ± 0.0030 | 0.0096 ± 0.0028 | 0.0065 ± 0.0022 |
| ATP 1 n = 12 | 0.0887 ± 0.0135 | 0.1164 ± 0.0196 | 0.0342 ± 0.0093 |
| PKA subunit n = 11 | 0.1087 ± 0.0166 | 0.1256 ± 0.0222 | 0.0822 ± 0.0321 |
| PKG subunit n = 8 | 0.0724 ± 0.0118 | 0.0522 ± 0.0173 | 0.0504 ± 0.0133 |
| PKC subunit n = 10 | 0.0443 ± 0.0075 | 0.0277 ± 0.0064 | 0.0253 ± 0.0060 |
| PKC inhib n = 6 | 0.0740 ± 0.0180 | 0.0208 ± 0.0055 | 0.0400 ± 0.0087 |
| Staurosporine n = 6 | 0.0468 ± 0.0149 | 0.0505 ± 0.0147 | 0.0375 ± 0.0097 |
| Okadaic acid n = 7 | 0.3075 ± 0.0581 | 0.4422 ± 0.0403 | 0.3992 ± 0.0962 |
Experimental values of BK single channel open probability (Popen) for GH3, GH4, and GH4 STREX cells. ATP0 (no ATP added to the patch pipette); ATP (1 mM added to the patch pipette; all further solutions contained 1 mM ATP during application of PKA, protein kinase A; PKG, protein kinase G; PKC, protein kinase C; STS, staurosporine; OA, okadaic acid; Po-values are presented as mean ± standard error of mean, and n is the number of patches/cells.