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. Author manuscript; available in PMC: 2014 Nov 12.
Published in final edited form as: Methods Mol Biol. 2013;951:57–67. doi: 10.1007/978-1-62703-146-2_5

Fig. 3.

Fig. 3

(a) GlcNAlk was metabolically incorporated into NIH-3T3 cells. Following lysis in 4% SDS, soluble proteins were subjected to CuAAC with azido-azo-biotin. Affinity enrichment using streptavidin beads isolated GlcNAlk-modified proteins. The proteins were then liberated from the beads using sodium dithionite (50 mM), separated by SDS-PAGE and subjected to proteolysis. (b) Inputs and biotin enriched proteins stained with Coomassie blue. *Large smear at bottom of the gel is streptavidin. LC-MS proteomics were conducted to identify 374 GlcNAlk-modified proteins of high and medium confidence (10).