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. Author manuscript; available in PMC: 2014 Nov 12.
Published in final edited form as: Fly (Austin). 2008 Nov 15;2(6):306–309. doi: 10.4161/fly.7427

Figure 1.

Figure 1

Genetic cross to produce rumi26 mutant embryos expressing activated Ras (RasV12). Primary cultures were established from a mixture of embryos of all four genotypes. The boxed embryos are the desired genotype. The UAS-RasV12 insertion site was mapped to 3R:7,394,981 (it is accompanied by a 151 bp deletion) within the 5′ region of CG14709. The Act-GAL4 insertion site was mapped to 3R:25,584,989 between CG1973 and kayak. The Act5C-GAL4 transgene is inserted in sequence corresponding to a transposable element and is currently being mapped further genetically.