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. 2014 Aug 13;7(11):1653–1669. doi: 10.1093/mp/ssu088

Figure 4.

Figure 4

AGL21 Expression Is Regulated by Hormones.

(A–C) qRT–PCR analyses of AGL21 expression in wild-type seedlings during the time course after IAA (A), MeJA (B), or ABA (C) treatment. Eight-day-old Col-0 seedlings were incubated in MS liquid cultures with 10 μM IAA, 50 μM MeJA, and 20 μM ABA, respectively, and whole seedlings were harvested at indicated time points for RNA extraction and qRT–PCR analyses. The transcript levels of AGL21 were normalized to the UBQ5 expression. Values are mean ± SD and asterisks denote Student’s t-test significance compared with untreated plants: * P < 0.05; ** P < 0.01; *** P < 0.001.

(D, E) IAA, MeJA, and ABA-induced pAGL21::GUS expression in the primary root (D), LRP, and LR (E). Seven-day-old seedlings of pAGL21::GUS transgenic line grown on MS agar medium were transferred either to hormone-free MS agar medium or to MS agar medium supplemented with 10 μM IAA, 10 μM MeJA, or 10 μM ABA for 1 d, respectively. The seedlings were harvested for GUS staining for 8h.