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. 2014 Aug 13;7(11):1653–1669. doi: 10.1093/mp/ssu088

Figure 5.

Figure 5

Response of AGL21 to Multiple Environmental Stresses.

(A) Response of AGL21 to N starvation. Seven-day-old Col-0 seedlings were transferred to N-free nutrient solution and harvested at indicated time points for RNA extraction and qRT–PCR analyses.

(B) Response of AGL21 to S starvation. Seven-day-old Col-0 seedlings were transferred to S-free agar medium for vertical growth. Complete nutrient medium was used as control. Roots were harvested at indicated time points for RNA extraction and qRT–PCR analyses.

(C) qRT–PCR analyses of AGL21 expression in 8-day-old Col-0 seedlings after 2.5h of drought or 120mM NaCl treatment. The transcript levels of AGL21 were normalized to the UBQ5 expression. Values are mean ± SD of three replica experiments and asterisks denote Student’s t-test significance compared with the 0-h control plants: * P < 0.05; ** P < 0.01; *** P < 0.001.

(D–F) Effects of N deprivation and S deprivation on pAGL21::GUS expression in the roots. Expression of AGL21 is induced by N deprivation and S deprivation in the PR tips (D), differential zone (E) after 1–4 d of N or S starvation, and LRPs after 3 d of N or S starvation (F). Five-day-old pAGL21::GUS transgenic seedlings grown on MS medium were transferred to N- or S-free medium for 1–4 d and seedlings were harvested at the indicated time points for GUS staining for 8h.