AGL21 Is Important for Sustaining LR Development under Low-N Conditions.
Five-day-old seedlings grown on MS medium were transferred to N-free medium and grown vertically. During the vertical growth stage, the root morphological parameters were measured.
(A) Phenotype of 11-day-old seedlings of AGL21-overexpressing, Col-0, and AGL21 knockout (bar = 1cm).
(B, C) Numbers of visible lateral roots of AGL21-overexpressing, Col-0, and AGL21 knockout plants on MS medium (B) and N-free medium (C) from the 7th day to the 10th day. Values are mean ± SD of three independent experiments each containing 15–20 plants per genotype.
(D) Average PR length of the 11-day-old plants. Values are mean ± SD of three independent experiments each containing 15–20 plants per genotype.
(E–G) Length of visible LRs per cm PR length (E), LR density (F), and average LR length (G). Average LR length defined as the ratio of total LR length over LR number. Values are mean ± SD of three independent experiments each containing 15–20 plants per genotype and asterisks denote Student’s t-test significance compared with the wild-type plants: * P < 0.05; ** P < 0.01; *** P < 0.001.