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. 2014 Aug 13;7(11):1653–1669. doi: 10.1093/mp/ssu088

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© The Author 2014. Published by Oxford University Press on behalf of CSPB and IPPE, SIBS, CAS.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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AGL21 Increases Auxin Accumulation in the Root through Local Biosynthesis.

(A) Effects of auxin transport inhibitors NPA on LR initiation in wild-type, AGL21-overexpressing and mutant plants. Five-day-old seedlings were transferred to medium supplemented with DMSO alone or the auxin transport inhibitor NPA (0.5 μM) dissolved in DMSO. After 7 d of growth, the LRs produced in the new growth were counted on 30 seedlings. Values are mean ± SD of three replica experiments and asterisks denote Student’s t-test significance compared with the wild-type plants: * P < 0.05; ** P < 0.01.

(B, C) Relative expression levels of auxin transport genes (B) and auxin biosynthesis genes (C) in the roots of 9-day-old plants. The transcript levels of auxin transport or biosynthesis genes were normalized to the UBQ5 expression. The expression levels of each gene in the wild-type were set as 1.0. Values are mean ± SD of three replica experiments and asterisks denote Student’s t-test significance compared with the wild-type plants: * P < 0.05; ** P < 0.01.