Figure 3.
3D EM Reconstruction of the Proteins on the Membrane
(A and B) Central and tangential slices through a tomogram of MPEEVs displaying gB (A) and EFF-1 (B). Scale bar represents 50 nm.
(C and D) Isosurface representation of the sub-volume reconstruction of gB with the trimer crystal structure (Protein Data Bank [PDB]: 2GUM) fitted (C) and EFF-1 EM map with a protomer of EFF-1 crystal structure (PDB: 4OJC) flexibly fitted (D), side (left) and top views (right) are presented. Membrane is shown in light blue; protein in orange. Scale bar represents 5 nm.
(E and F) Isosurface representations of the tomograms shown in (A and B). The subvolume reconstruction and the membrane were placed back into the determined position and orientation of individual protein spikes such enabling analysis of relative orientations and interactions. Scale bar represents 50 nm.