Table 1.
Major Proteins Identified by Mass Spectrometry in the Vesicles Preparations and Their Relative Abundance
| Protein Namea | NCBI Accession | Mass (Da) | HSV1 gB |
AFF-1 |
EFF-1 |
||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Scoreb | emPAIc | Rel. Abund.d (%) | Scoreb | emPAIc | Rel. Abund.d (%) | Scoreb | emPAIc | Rel. Abund.d (%) | |||
| Envelope glycoprotein B (HSV1) | 1353200 | 100,875 | 16,873 | 70.91 | 27.6 | ||||||
| Protein AFF-1 anchor cell fusion failure-1 (C. elegans) | 193204255 | 68,617 | 974 | 2.07 | 33 | ||||||
| Protein EFF-1, isoform a (C. elegans) | 71982882 | 75,494 | 1004 | 1.66 | 16 | ||||||
| Histone H2B homolog | 156371481 | 24,545 | 225 | 1.78 | 28 | 392 | 1.78 | 17 | |||
| Actin familye | 178045 | 26,147 | 2,918 | 46.51 | 18.1 | 221 | 1.05 | 17 | 185 | 0.35 | 3 |
| Hemoglobin fetal subunit beta | 62460494 | 15,963 | 998 | 33.03 | 12.9 | 95 | 0.78 | 12 | 234 | 1.62 | 15 |
| Pyruvate kinase PKM | 146345448 | 58,378 | 2,081 | 18.19 | 7.0 | ||||||
| Tubulin beta-3 chain | 12963615 | 50,842 | 1,422 | 17.00 | 6.6 | 134 | 0.37 | 3 | |||
| 14-3-3 protein zeta | 82197807 | 27,929 | 625 | 16.42 | 6.4 | ||||||
| Ras-related protein Rap | 75077355 | 21,040 | 356 | 13.03 | 5.1 | ||||||
| Annexin A2 | 113951 | 38,937 | 1,730 | 12.89 | 5.0 | ||||||
| T-complex protein 1 subunit zeta | 115305833 | 58,376 | 405 | 10.15 | 4.0 | ||||||
| Class-III intermediate filaments | 138535 | 53,754 | 1,500 | 9.64 | 3.8 | ||||||
| Tubulin alpha | 116256086 | 50,804 | 1,614 | 9.10 | 3.5 | ||||||
| Histone cluster 1, H2ag-like | 291410763 | 27,347 | 141 | 1.00 | 10 | ||||||
| Serum albumin | 1351907 | 71,244 | 152 | 0.20 | 3 | 282 | 0.31 | 3 | |||
| Galectin-3-binding protein | 81861611 | 65,270 | 272 | 0.41 | 7 | 315 | 0.63 | 6 | |||
| 60S acidic ribosomal protein P2 | 133062 | 4,692 | 72 | 0.79 | 8 | ||||||
| 40S ribosomal protein SA-like | 296190805 | 32,906 | 182 | 0.47 | 4 | ||||||
| Guanine nucleotide-binding protein subunit beta-2-like 1 | 5174447 | 35,511 | 170 | 0.56 | 5 | ||||||
| Glyceraldehyde-3-phosphate dehydrogenase-like | 488563203 | 35,942 | 190 | 0.55 | 5 | ||||||
| Laminin-binding protein | 34234 | 31,888 | 197 | 0.49 | 5 | ||||||
All proteins other than gB of HSV1 and AFF-1 and EFF-1 of C. elegans are originating either from the BHK cells used to produce the vesicles or contaminations, either from the serum added to the cell culture or transfection reagent.
Mascot score for confidence of protein identification is defined as the –log value of the probability P that this assignment is made by chance (Mackeen et al., 2010).
Exponentially modified protein abundance index (emPAI) analysis (Ishihama et al., 2005, Trudgian et al., 2011).
Relative abundance (Rel. Abund.) in respect to the proteins with the highest emPAI listed in this table.
Actin family representing gamma-actin, cytoplasmic actin 2, actin-cytoplasmic 1-like.