Skip to main content
. 2014 Oct 7;15(11):4046–4053. doi: 10.1021/bm501091p

Figure 4.

Figure 4

(a) GAA activity assay after 48 h incubation of the GAA loaded nanogel at pH 5.0 and pH 7.4: monitoring absorbance at 400 nm of the pNP produced due to cleavage of the α-1,4-linkages of para-nitrophenol-α-d-glucopyranoside substrate. (b) Comparison of the end point activity; protein concentration in each case = 0.173 μM; temperature = 37 °C. (c) Time variable DLS profile (PDI = 0.354, 0.44, 0.40, 0.45) of native GAA and GAA loaded in the nanogel. (d) TEM image of GAA encapsulated NG; inset shows zoomed NG.