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Schematic representation of preparation of fluorophore-labeled FXa. FX was first labeled with donor (Alexa Fluor 555 succinimidyl ester; A555-NHS) followed by activation to remove the activation peptide at its N-terminus. The acceptor (fluorescein-labeled EGR-chloromethylketone; FEGRck) was then added to the active site of A555-Xa to produce doubly labeled peptide or to FXa to produce singly labeled peptide.
