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. 2014 Nov 12;9(11):e111566. doi: 10.1371/journal.pone.0111566

Figure 3. Chondrogenesis of iPSCs cultured on scaffolds (scaffold group) or plates (control group) in vitro.

Figure 3

Collagen II synthesis in the scaffold group (A,B,C) and control group (D,E,F) was analyzed by immunofluorescence staining, including nuclear counterstaining with DAPI (A,D), anti-collagen II staining in red (B,E); merged images are also shown (C,F). (G) qRT-PCR analysis of gene expression and (H) western blotting for collagen II, aggrecan, sox9 and collagen I, using GAPDH as the internal reference. The nanofibrous scaffolds generally enhanced cartilage-specific gene expression and protein levels. ♦ Statistically significant difference compared with the control group (p<0.05); • Statistically significant difference compared with the control group (p<0.01).