Figure 1. Cartoon models depicting a recombinant membrane tethered ligand (panel A) and a corresponding synthetic membrane anchored ligand (panel B).

Note: Linker₂ differs between tSubP and tCCK4 constructs. Tethered Substance P sequence is as shown, while the Linker₂ sequence for tCCK4 constructs is GN(4)S(1)GS(9). Abbreviations: TNFα TMD = Tumor necrosis factor α transmembrane domain. Amino acids are represented in single letter code.