Figure 6. The inhibitory dose response of 4-acetylpyridine on metamorphosis of C. teleta after 24 h.

Larvae were acclimated to the listed concentrations of 4-acetylpyridine (4-ACP) for one hour before being transferred to a solution of the same concentration of 4-acetylpyridine and pyrazinecarboxamide at 40 µM. Each treatment consisted of 3 replicates with 8 larvae per replicate. Artificial seawater (Instant Ocean) acted as a negative control. The PZC treatment acted as a positive control containing only 40 µM pyrazinecarboxamide and artificial seawater. Letters indicate significant (P<0.05) differences as determined by a Bonferroni post-hoc test. Error bars represent +1 s.e.m.