FIGURE 2.
Ex vivo CAM model allowing for fetal wound manipulation. Procuring fetal skin, implantation onto the CAM, and laser wound injury is depicted. A, Fertilized chicken egg with a 1.5 × 1 cm oval window for visualization of grafts and tissue reaction. B, E16.5 mouse fetus. C, Dorsal skin flap is elevated on E16.5 fetus. D, Skin flap is excised prior to grafting. E, Donor fetal skin graft is transplanted onto the CAM. F, Oval window is covered with clear scotch tape. G, Fetal skin xenograft 2 hours after laser injury, and 48 hours post transplantation onto the CAM.