Table 1. Expression of genes responsible for motility and biofilm formation in E. coli CFT073 wild type after 48 h of growth in presence and absence of PIP and RES.
Piperine | Reserpine | |||
0.5 µg/mL | 5 µg/mL | 0.5 µg/mL | 5 µg/mL | |
fliC | 0.90 (0.4) | 0.68 (0.19) | 0.74 (0.19) | 0.91 (0.25) |
motA | 4.1×10−5 (5×10−2) | 0.030 (0.01) | 8.4×10−6 (1×10−3) | 1.2×10−6 (5×10−3) |
motB | 0.45 (0.09) | 0.38 (0.05) | 0.55 (0.19) | 0.48 (0.19) |
fimA | 1.4 (0.03) | 3.4 (0.17) | 2.1 (0.26) | 5.2 (0.35) |
uvrY | 1.6 (0.15) | 2.7 (0.40) | 1.1 (0.30) | 2.4 (0.5) |
papA | 1.1 (0.15) | 1.2 (0.17) | 0.89 (0.40) | 2.5 (0.35) |
Relative mRNA quantities were normalized to that of a housekeeping gene, gapA. Results represent mean fold change values ± SD (in parentheses) for three independent experiments with respect to control. Values in bold font indicate statistically significant differences in mRNA relative value with respect to the control (p<0.05).