Figure 6. Eupatorin induces ROS generation and scavengers of ROS inhibit cell death.

(A) HL-60 and Molt-3 cells were incubated with 10 µM eupatorin for 24 h and 6 h, respectively, and the fluorescence of oxidized H₂-DCF or DHE was determined by flow cytometry. (B) Molt-3 cells were incubated with eupatorin for 6 h and the H₂-DCF-derived fluorescence was measured as above. (C) Molt-3 cells were preincubated with N-acetyl-l-cysteine (NAC, 10 mM), trolox (2 mM) and vitamin E (25 µM) for 1 h and then incubated with eupatorin (10 µM) for 24 h and apoptotic cells were quantified by flow cytometry. Values represent means ± SE of two independent experiments, each performed in triplicate. * indicates P<0.05 for comparison with untreated control, # indicates P<0.05 for comparison with eupatorin treatment alone.