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. 2014 Nov 12;9(11):e112482. doi: 10.1371/journal.pone.0112482

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© 2014 De Maio et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A) Immunoblot analysis on whole cell lysates of Mtb expressing PE_PGRS30 and its HA-tagged chimeras with anti-C-terminal domain primary antibody. B) Immunoblot analysis on cytoplasmatic fraction of Mtb expressing PE_PGRS30 and its HA_tagged chimeras probed with anti-HA antibody, anti-C-terminal domain and anti-MPT64 sera; 1: Mtb-PE_PGRS30^HA; 2: Mtb-₃₀PE_CT^HA; Arrows indicate the band corresponding to PE_PGRS30^HA and PE_CT^HA and PE_PGRS62.