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. 2014 Nov 12;9(11):e112529. doi: 10.1371/journal.pone.0112529

Figure 5. Effects of nicorandil and LiCl on GSK-3β phosphorylation.

Figure 5

A: Representative Western blotting for Ser9-phospho-GSK-3β and total GSK-3β in cytosolic and mitochondrial fractions of vehicle-treated, nicorandil-treated (NC) and 5-hydroxydecanoate (5-HD) plus NC-treated cells. B: Group means of mitochondrial Ser9-phospho-GSK-3β levels. NC and 5-HD were added to the culture medium 60 min and 90 min before collection of cells for Western boltting, respectively. *p<0.05 vs. Vehicle, †p<0.05 vs. NC+Vehicle. N = 8 per group. C: Effects of MPG (mercaptopropionyl glycine) on the effect of NC-induced phosphorylation of GSK-3β.