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. 2014 Nov 12;9(11):e112284. doi: 10.1371/journal.pone.0112284

Figure 3. pSP-CS-mediated human IGF-1 and IL-1Ra gene expression levels in cell supernatants and synovial fluid from rabbit knees.

Figure 3

(A) The concentration of IGF-1 in the cell supernatant and synovial fluid was analyzed by ELISA. The data are shown as the mean ±S.D. A similarly high level of IGF-1 expression was detected in the pBudCE4.1-IL-1Ra+igf-1/ pSP-CS and pBudCE4.1-igf-1/ pSP-CS transfection groups. No significant difference was observed among the pBudCE4.1-IL-1Ra/ pSP-CS, pBudCE4.1/ pSP-CS and control groups. (B) The concentration of IL-1Ra in the cell supernatant and synovial fluid was analyzed by ELISA. The data are shown as the mean ± S.D. A similarly high level of IL-1Ra expression was detected in the pBudCE4.1-IL-1Ra+igf-1/ pSP-CS and pBudCE4.1-IL-1Ra/ pSP-CS transfection groups. No significant difference was observed among the pBudCE4.1-igf-1/ pSP-CS, pBudCE4.1/ pSP-CS and control groups.