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. 2014 Nov 12;9(11):e112759. doi: 10.1371/journal.pone.0112759

Figure 8. Cadmium tolerance of E. coli BL21 cells expressing OcMT1 and OcMT2.

Figure 8

A: OcMT1, B: OcMT2. Bacterial growth curve of E. coli cells transformed with pET-28a, pET-28a-OcMT and pET-28a-OcMT-IPTG. pET-28a is an “empty” vector; pET-28a-OcMT group is transformed with the OcMT1 or OcMT2 gene without IPTG; pET-28a-OcMT-IPTG group is transformed with the OcMT1 or OcMT2 gene with IPTG. Five microliters of CdCl2 was added into medium when bacteria were grown to OD600 = 0.6. All bacteria were grown for 11 h. Concentration gradient of CdCl2 were 0, 0.82, 1.74, 3.27 mM.