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. 2014 Nov 12;9(11):e112712. doi: 10.1371/journal.pone.0112712

Figure 1. Transposon fleet.

Figure 1

Schematic representation of the generated plasmids. SB100X carries the Hyperactive Sleeping Beauty transposase coding sequence placed under the control of the CMV promoter and followed by an SV40 poly-Adenylation (pA) signal. The transposons T2 and SA possess the expression cassette consisting of the CAGGS promoter, VENUS reporter gene and SV40 pA signal. The stuffer DNA represented has variable increasing size. The arrows represent the IR/DR ends recognised by the transposase. SA constructs are characterized by the presence of two complete IR/DR at each ends (white and black arrows) and the asterisks underline the IR mutated site not recognized as a catalytic substrate by the transposase. Numbers following T2 or SA abbreviation indicate the size in kilobases of the transposed cassette.