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. 2014 Nov 12;9(11):e112762. doi: 10.1371/journal.pone.0112762

Figure 4. Phosphorylation and acetylation do not alter the ability of HBZ to repress Tax transactivation of the LTR promoter.

Figure 4

(A) 293 T cells were transfected with LTR-1-luc reporter (100 ng), renilla-TK (20 ng), S-tagged Tax (200 ng) and a titration of HBZ mutants (100 ng and 500 ng). DNA amounts were normalized with empty Flag-6xHis plasmid. Twenty-four hours post-transfection, lysates were collected and luciferase levels were measured. (B) Jurkat T-cells were transfected with LTR-1-luc reporter (300 ng), renilla-TK (100 ng), S-tagged Tax (600 ng) and HBZ mutants (1500 ng). Forty hours post-transfection, lysates were collected and luciferase levels were measured. Error bars represent standard deviations.