Figure 6. Phosphorylation and acetylation do not affect the ability of HBZ to inhibit c-Jun-mediated transcription.

293 T cells were transfected with a 6xAP-1-luc reporter (50 ng), renilla-TK (10 ng), pCMV-c-Jun (25 ng) and a titration of HBZ constructs (10 ng and 100 ng). DNA amounts were normalized with empty Flag-6xHis plasmid. Lysates were collected 24 hours post-transfection and luciferase levels were measured. Error bars represent standard deviations.