Figure 2. Cuvette-based set-up for combined O2 exchange and variable chlorophyll fluorescence measurements on Symbiodinium cultures.
The O2 optode was inserted into the cuvette through a small hole in the gas-tight lid. Actinic light was provided by the OL-490 light engine equipped with a liquid light guide and collimator. The MC-PAM provided measuring light in opposite position to the actinic light and at right angles to the fluorescence detector. The cuvette temperature was controlled at 25°C by an ambient water bath and heater.