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. 2014 Nov 12;9(11):e112689. doi: 10.1371/journal.pone.0112689

Figure 4. TJ-84 reduced 5-FU-induced cell death.

Figure 4

(A), Cytotoxicity of TJ-84. Sa3 cells were incubated with various concentrations of TJ-84 for 24 h, and cell viability was then measured using WST-8 kits. Values are means ± S.E.M. (n = 3). **p<0.01 compared to control cells that had not been incubated with TJ-84. (B), Viability of cells incubated with various concentrations of TJ-84 for 1 h and then incubated with 5-FU for 24 h. Results are expressed as percentages with respect to control cells that had not been incubated with TJ-84 and 5-FU. Values are means ± S.E.M. (n = 4). **p<0.01 compared to control cells. (C), The effect of TJ-84 on LDH release from cells incubated with 5-FU for 24 h was assessed using WST-8 kits. The supernatant of Sa3 cells incubated with 0.1% Triton-X for 5 min was used as a positive control (Triton). The LDH levels in the supernatants are expressed as percentages with respect to cells that had not been incubated with 5-FU. Values are means ± S.E.M. (n = 4). *p<0.05 compared to the control cells.