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. 1973 Nov;8(5):762–768. doi: 10.1128/iai.8.5.762-768.1973

Synergistic Effect in Viral-Bacterial Infection: Combined Infection of the Murine Respiratory Tract with Sendai Virus and Pasteurella pneumotropica

George J Jakab a,1, Elliot C Dick a
PMCID: PMC422924  PMID: 4356072

Abstract

Synergism was demonstrated between Sendai virus and Pasteurella pneumotropica in the respiratory tract of mice showing no evidence of previous infection with either agent. Mice aerosol challenged with P. pneumotropica invariably eliminated the viable organism from their lungs within 72 h. In contrast, intrapulmonary killing was delayed in animals previously infected with Sendai virus. Maximum synergism was observed when virus infection preceded bacterial challenge by 6 days. At this time, a mortality rate of 37% was observed as compared with 0, 10, 20, and 10%, respectively, in those animals in which the virus infection preceded bacterial challenge by 1, 3, 9, and 12 days. Previous immunization with Sendai virus completely prevented virus infection and thus the synergistic effect. Synergism with endogenous flora was also noted. Six days after virus infection an endogenous Pasteurella sp. began to proliferate in the bronchopulmonary tissues. Up to 104 colony-forming units per lung were recovered but no animals died of the endogenous Pasteurella infection.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Appell L. H., Kovatch R. M., Reddecliff J. M., Gerone P. J. Pathogenesis of Sendai virus infection in mice. Am J Vet Res. 1971 Nov;32(11):1835–1841. [PubMed] [Google Scholar]
  2. Brennan P. C., Fritz T. E., Flynn R. J. Pasteurella pneumotropica: cultural and biochemical characteristics, and its association with disease in laboratory animals. Lab Anim Care. 1965 Oct;15(5):307–312. [PubMed] [Google Scholar]
  3. Brennan P. C., Fritz T. E., Flynn R. J. Role of Pasteurella pneumotropica and Mycoplasma pulmonis in murine pneumonia. J Bacteriol. 1969 Jan;97(1):337–349. doi: 10.1128/jb.97.1.337-349.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Buddingh G. J., al-Talib A. M., Pipes F. J. Combined viral and bacterial infection. An in vitro analysis of the population dynamics and factors influencing the enhancement of virulence of hemophilus influenzae in combined infection with influenza virus in embryonated eggs. Am J Pathol. 1966 Aug;49(2):353–363. [PMC free article] [PubMed] [Google Scholar]
  5. Degré M., Glasgow L. A. Synergistic effect in viral-bacterial infection. I. Combined infection of the respiratory tract in mice with parainfluenza virus and Hemophilus influenza. J Infect Dis. 1968 Dec;118(5):449–462. doi: 10.1093/infdis/118.5.449. [DOI] [PubMed] [Google Scholar]
  6. GERONE P. J., WARD T. G., CHAPPELL W. A. Combined infections in mice with influenza virus and Diplococcus pneumoniae. Am J Hyg. 1957 Nov;66(3):331–341. doi: 10.1093/oxfordjournals.aje.a119906. [DOI] [PubMed] [Google Scholar]
  7. GRAY D. F., CAMPBELL A. L. The use of chloramphenicol and foster mothers in the control of natural pasteurellosis in experimental mice. Aust J Exp Biol Med Sci. 1953 Apr;31(2):161–165. doi: 10.1038/icb.1953.19. [DOI] [PubMed] [Google Scholar]
  8. GREEN G. M., KASS E. H. THE ROLE OF THE ALVEOLAR MACROPHAGE IN THE CLEARANCE OF BACTERIA FROM THE LUNG. J Exp Med. 1964 Jan 1;119:167–176. doi: 10.1084/jem.119.1.167. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Goldstein E., Green G. M. Alteration of the pathogenicity of Pasteurella pneumotropica for the murine lung caused by changes in pulmonary antibacterial activity. J Bacteriol. 1967 May;93(5):1651–1656. doi: 10.1128/jb.93.5.1651-1656.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Green G. M. Pulmonary clearance of infectious agents. Annu Rev Med. 1968;19:315–336. doi: 10.1146/annurev.me.19.020168.001531. [DOI] [PubMed] [Google Scholar]
  11. HERS J. F., MUDLER J., MASUREL N., vd KUIP L., TYRRELL D. A. Studies on the pathogenesis of influenza virus pneumonia in mice. J Pathol Bacteriol. 1962 Jan;83:207–217. [PubMed] [Google Scholar]
  12. HEYL J. G. A study of Pasteurella strains from animal sources. Antonie Van Leeuwenhoek. 1963;29:79–83. doi: 10.1007/BF02046040. [DOI] [PubMed] [Google Scholar]
  13. JANSSEN R. J., CHAPPELL W. A., GERONE P. J. SYNERGISTIC ACTIVITY BETWEEN PR8 INFLUENZA VIRUS AND STAPHYLOCOCCUS AUREUS IN THE GUINEA PIG. Am J Hyg. 1963 Nov;78:275–284. [PubMed] [Google Scholar]
  14. JAWETZ E. A pneumotropic pasteurella of laboratory animals; bacteriological and serological characteristics of the organism. J Infect Dis. 1950 Mar-Apr;86(2):172–183. doi: 10.1093/infdis/86.2.172. [DOI] [PubMed] [Google Scholar]
  15. JAWETZ E., BAKER W. H. A pneumotropic pasteurella of laboratory animals; pathological and immunological studies with the organism. J Infect Dis. 1950 Mar-Apr;86(2):184–196. doi: 10.1093/infdis/86.2.184. [DOI] [PubMed] [Google Scholar]
  16. Jakab G. J., Green G. M. The effect of Sendai virus infection on bactericidal and transport mechanisms of the murine lung. J Clin Invest. 1972 Aug;51(8):1989–1998. doi: 10.1172/JCI107005. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Klein J. O., Green G. M., Tilles J. G., Kass E. H., Finland M. Effect of intranasal reovirus infection on antibacterial activity of mouse lung. J Infect Dis. 1969 Jan;119(1):43–50. doi: 10.1093/infdis/119.1.43. [DOI] [PubMed] [Google Scholar]
  18. Loosli C. G. Synergism between respiratory viruses and bacteria. Yale J Biol Med. 1968 Apr-Jun;40(5-6):522–540. [PMC free article] [PubMed] [Google Scholar]
  19. PARKER J. C., TENNANT R. W., WARD T. G., ROWE W. P. ENZOOTIC SENDAI VIRUS INFECTIONS IN MOUSE BREEDER COLONIES WITHIN THE UNITED STATES. Science. 1964 Nov 13;146(3646):936–938. doi: 10.1126/science.146.3646.936. [DOI] [PubMed] [Google Scholar]
  20. Parker J. C., Reynolds R. K. Natural history of Sendai virus infection in mice. Am J Epidemiol. 1968 Jul;88(1):112–125. doi: 10.1093/oxfordjournals.aje.a120859. [DOI] [PubMed] [Google Scholar]
  21. Robinson T. W., Cureton R. J., Heath R. B. The pathogenesis of Sendai virus infection in the mouse lung. J Med Microbiol. 1968 Aug;1(1):89–95. doi: 10.1099/00222615-1-1-89. [DOI] [PubMed] [Google Scholar]
  22. SELLERS T. F., Jr, SCHULMAN J., BOUVIER C., McCUNE R., KILBOURNE E. D. The influence of influenza virus infection on exogenous staphylococcal and endogenous murine bacterial infection of the bronchopulmonary tissues of mice. J Exp Med. 1961 Aug 1;114:237–256. doi: 10.1084/jem.114.2.237. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Sawyer W. D. Interaction of influenza virus with leukocytes and its effect on phagocytosis. J Infect Dis. 1969 Jun;119(6):541–556. doi: 10.1093/infdis/119.6.541. [DOI] [PubMed] [Google Scholar]
  24. Tyrrell D. A., Coid C. R. Sendai virus infection of rats as a convenient model of acute respiratory infection. Vet Rec. 1970 Feb 7;86(6):164–165. doi: 10.1136/vr.86.6.164. [DOI] [PubMed] [Google Scholar]

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