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. 2014 Nov 12;9(11):e112904. doi: 10.1371/journal.pone.0112904

Figure 2. Bacterial stimulation-mediated reappearance of FV3 DNA in PLs of adult frogs after viral clearance.

Figure 2

(A) Experimental outline. (B) PCR assay on total DNA purified from PLs harvested from 15 different outbred frogs (Experiment 3 in Table 2) at 30 dpi, and (C) PCR assay on DNA purified from PLs collected from the same animals 3 days after bacterial stimulation (35 dpi). Presence of viral DNA was assed by PCR on 50 ng of total DNA using FV3 specific primers for MCP and vDNA poly II as well as X. laevis Ef-1α as a loading control.