Figure 3. Detection of viral gene expression in PLs of frogs following bacterial stimulation.

RT-PCR assay of DNase-treated RNA purified from PLs harvested from the same 15 different frogs used in Fig. 2 ((Experiment 3 in Table 2) at 30 dpi (A), and 3 days later after bacterial stimulation at 35 dpi (B) using FV3 specific primers for vDNA pol and MCP as well as X. laevis Ef-1α as a loading control. RT minus controls were included to rule out contamination by genomic DNA.