Figure 5. PCR diagnostic of asymptomatic FV3 infected adult frogs that died following bacterial stimulation.

PCR was performed on DNA extracted from spleen (Spl), liver (Liv) and kidneys (Kid) of a representative of frogs that died 15, 18 or 29 days after bacterial stimulation (45, 48 or 59 dpi, respectively) using primers specific for FV3 MCP, vDNA poly II and EF-1α as control.