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. 2014 Nov 12;9(11):e112904. doi: 10.1371/journal.pone.0112904

Figure 8. Detection of infectious virus in kidneys but not PLs of reactivated asymptomatic infection.

Figure 8

(A) PCR assay on total DNA purified from PLs harvested from 10 different outbred frogs at 30 dpi and 35 dpi, (3 days after bacterial stimulation). Presence of viral DNA was assed by PCR on 50 ng of total DNA using FV3 specific primers for MCP as well as X. laevis Ef-1α as a loading control. (B) Number of infectious FV3 (PFU/ml/whole kidney) detected in kidney by plaque assay for frogs number (#) 3, 5, 6, 7, 10 from A sacrificed at 35 dpi. (C) Number of infectious FV3 detected by plaque assay from PLs and kidneys of FV3 infected frogs at 3, 6 and 33 dpi (3 individual for each time point).