Table 2. Detection of viral DNA and viral transcription in X. laevis adult's PL cells lysates after FV3 infection or FV3 + Bacterial stimulation.
| Treatment a | Experiments | Total Number of frogs | PCR b | RT-PCR b | Sign of infection | Death c |
| FV3 + E. coli | Exp. 1 | 10 | 7 | 7 | 2 | 3 (30%) |
| Exp. 2 | 10 | 5 | 5 | 4 | 5 (50%) | |
| Exp. 3 | 15 | 11 | 11 | 5 | 7 (43%) | |
| Exp. 4 | 10 | 5 | 2 | 2 | 1 (10%) | |
| Total | 45 | 28 | 25 | 13 | 16 (36%) | |
| FV3 only | Exp. 1 | 10 | 3 | 3 | ND | 0 (0%) |
| Exp. 2 | 10 | 1 | 0 | ND | 0 (0%) | |
| Exp. 3 | 20 | 2 | 3 | 3 | 4 (20%) | |
| Exp. 4 | 10 | 3 | 0 | ND | 0 (0%) | |
| Total | 50 | 9 | 6 | 3 | 4 (8%) | |
| E. Coli only | Exp. 1 | 10 | ND | ND | 1 | 1 (10%) |
| Exp. 2 | 10 | ND | ND | ND | 1 (10%) | |
| Exp. 3 | 13 | ND | ND | ND | 0 (0%) | |
| Total | 33 | ND | ND | 1 | 2 (6%) | |
| APBS | Exp. 1 | 5 | ND | ND | ND | 0 (0%) |
| Exp. 2 | 5 | ND | ND | ND | 0 (0%) | |
| Exp. 3 | 8 | ND | ND | ND | 0 (0%) | |
| Total | 18 | ND | ND | ND | 0 (0%) |
a
Treatments: Adult frogs were treated by i.p. injection with: APBS only (30 days treatment); E. coli only (3 days treatment); FV3 only (30 days treatment); or FV3 + E. coli (30 days treatment with FV3 preceding of 2 days of recovery and 3 days treatment with HK E. coli).
b
FV3 detection by the indicated method; ND: not detected.
c
Death that resulted from the treatments. All frogs in each group were monitored and death was recorded daily for 60 days after each treatment.