Table 1.
Intrinsic membrane properties
| PV-Rosa BC | BP-Rosa BiS | PV-Rosa SO | SOM-Rosa | YFP+ WT | YFP+ Homo | CA1 PC | PV-Rosa PFC | |
|---|---|---|---|---|---|---|---|---|
| Property | (n = 6) | (n = 13) | cells (n = 11) | (n = 15) | PV-M1KO (n = 8) | PV-M1KO (n = 9) | (n = 8) | (n = 7) |
| Rin (MΩ) | 66.0 ± 8.7*†‡§¶**†† | 100.0 ± 7.3¶** | 104.5 ± 5.6¶** | 272.9 ± 25.7†† | 113.8 ± 15.9¶** | 139.6 ± 22.2¶** | 176.8 ± 23.5** | 121.7 ± 13.4 |
| 95.6 ± 6.4§¶**†† | ||||||||
| First AP half-width (μs) | 261 ± 19†§¶**†† | 329 ± 20§¶**†† | 385 ± 30¶** | 639 ± 33†† | 315 ± 18§¶**†† | 496 ± 50¶** | 870 ± 32†† | 465 ± 33 |
| 366 ± 18§¶**†† | ||||||||
| First AP height (mV) | 45.3 ± 3.8¶ | 47.8 ± 3.7¶ | 59.0 ± 3.5 | 52.9 ± 2.0 | 55.6 ± 2.1¶ | 55.5 ± 3.4¶ | 73.4 ± 4.6**†† | 54.4 ± 2.9 |
| 51.4 ± 2.4¶ | ||||||||
| Cm (pF) | 115.8 ± 13.3§†† | 98.8 ± 4.0§†† | 88.9 ± 6.0 | 89.2 ± 7.4 | 84.6 ± 10.2 | 76.0 ± 10.0¶ | 138.7 ± 30.6 | 75.7 ± 9.4 |
| 98.6 ± 4.1§¶†† | ||||||||
| Sag ratio (SS/peak) | 1.0 ± 0.01¶§‡**†† | 1.0 ± 0.01¶§**†† | 0.95 ± 0.01** | 0.81 ± 0.03†† | 0.88 ± 0.06 | 0.77 ± 0.07†† | 0.92 ± 0.02†† | 0.97 ± 0.01 |
| 1.0 ± 0.0‡§¶** | ||||||||
| τ (ms) | 7.1 ± 0.2*†¶** | 9.3 ± 0.5¶** | 9.1 ± 0.9¶** | 22.9 ± 1.6†† | 9.0 ± 1.1¶** | 9.9 ± 1.4¶** | 21.1 ± 2.5†† | 8.9 ± 1.0 |
| 8.8 ± 0.4¶** | ||||||||
| Adaptation coefficient | 0.73 ± 0.10 | 0.66 ± 0.04¶ | 0.77 ± 0.05¶ | 0.69 ± 0.04†† | 0.56 ± 0.05†† | 0.66 ± 0.07 | 0.47 ± 0.08†† | 0.80 ± 0.05 |
| 0.7 ± 0.0‡¶ | ||||||||
| Frequency at 700 pA (Hz) | 223.3 ± 42.6§ | 163.6 ± 13.4§ | 195.4 ± 18.1§ | N.A. | 152.9 ± 17.8§ | 95.4 ± 4.8†† | N.A. | 184.4 ± 17.2 |
| 187.2 ± 12.4§ | ||||||||
| Ihold (pA) | −11.9 ± 42.3 | −25.4 ± 10.8¶§ | −43.8 ± 21.5 | −43.0 ± 12.2 | −61.0 ± 23.9¶†† | −68.9 ± 19.7¶†† | 66.8 ± 17.3** | 29.9 ± 35.1 |
| −30.6 ± 13.5¶ | ||||||||
Values are means ± S.E.M. Abbreviations: AP, action potential; BC, basket cell; BiS, bistratified cell; Homo, homozygous; M1KO, M1 muscarinic acetylcholine receptor knockout; PC, pyramidal cell; PFC, prefrontal cortex; PV, parvalbumin; SO, stratum oriens; SS, steady state; WT, wild-type. *P < 0.05 compared to PV-Rosa BiSs. †P < 0.05 compared to PV-Rosa SO cells. ‡P < 0.05 compared to YFP+ cells in WT PV-M1KO mice. §P < 0.05 compared to YFP+ cells in Homo PV-M1KO mice. ¶P < 0.05 compared to CA1 PCs. **P < 0.05 compared to SOM-Rosa cells. ††P < 0.05 compared to PFC PV cells.