Fig. 3. Effect of PP2 and PP3 on the ability of TPA or a physiological concentration of CCK (0.3 nM) to stimulate various kinases (Src, PYK2, p125FAK, paxillin and PKD).
Rat pancreatic acinar cells were pretreated with no additions or with PP2 (10 µM) or PP3 (10 µM) for 1 h and then incubated with 0.3 nM CCK or TPA (1 µM). The whole cell lysates were processed as described in the Figure 1 legend. Membranes were analyzed using anti-pY416 Src, pY402 PYK2, pY397 p125FAK, pY118 paxillin and pY744/748 PKD. Both a representative experiment of 3 others and the means of all the experiments are shown. * P< 0.05 vs control, # P< 0.05 vs PP2 alone, ∞ P< 0.05 vs PP3 alone and $ P< 0.05 comparing stimulants (CCK or TPA) vs stimulants pre-incubated with PP2 or PP3, respectively.