Rat pancreatic acinar cells were pre-incubated with 109 VP/ml of a control, nulled adenovirus or DN-Csk-Advirus for 6 h, which results in SFK activation. Subsequently, cell were incubated with no additions (control), with 0.3 nM CCK, 100 nM CCK for 2.5 min or with 1 µM TPA for 5 min and then lysed. Whole cell lysates and membranes were processed as indicated in Figure 1-legend. Membranes were analyzed using anti-pY416 Src, pY402 PYK2, pY397 p125FAK, and pY118 paxillin. Both a representative experiment of 3 others and the means of all the experiments are shown.* P< 0.05 vs control, # P< 0.05 vs DN-CSK-Ad-virus-control.