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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: J Cell Biochem. 2015 Jan;116(1):22–36. doi: 10.1002/jcb.24895

Fig. 7. Effect of pre-incubation with the SFK stimulating CSK dominant negative virus (DN-CSK-Advirus) on basal, CCK- and TPA-stimulated phosphorylation of kinases (p130CAS, Shc, p42/44, p38, JNK and GSK3-β).

Fig. 7

Effect of stimulation by CCK and TPA stimulation on the phosphorylation of p130CAS, Shc, P42/44, P38, JNK and GSK3-β in rat pancreatic acinar cells pre-incubated with 109 VP/ml of a nulled-Advirus or DN-CSK-Advirus (10 µM) for 6 h, which results in SFK activation. Subsequently, acini were incubated with no additions (control), with 0.3 nM CCK, 100 nM CCK for 2.5 min or with 1µM TPA for 5 min and then lysed. Whole cell lysates and membranes were processed as indicated in Figure 1-legend. Membranes were analyzed using anti-pY410 p130CAS, pY239/240 Shc, pY202/204 p42/44, pThr180/Y182 p38, pYThr183/Y185 JNK and pSer9 GSK3-β. Both a representative experiment of 3 others and the means of all the experiments are shown. * P< 0.05 vs control, # P< 0.05 vs DN-CSK-Ad-virus-control.