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. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: Ann Rheum Dis. 2014 Feb 23;74(6):1284–1292. doi: 10.1136/annrheumdis-2013-204782

Figure 1. IL-17A gene transfer induces myelopoiesis.

Figure 1

a) In vivo whole body and b) ex vivo 2D fluorescence imaging of C57BL/6 mouse liver explants one day post-gene transfer of IL-17A or GFP. Red denotes background and green GFP. c) Serum IL-17A levels, analyzed over 24 weeks showing stable expression after GFP or IL-17A MC transfer (p<0.0001, Mann-Whitney non-parametric test; data pooled from three experiments). Flow cytometric analysis of d) bone marrow cells and e) splenocytes derived from GFP MC or IL-17A MC at day 3 depicting CD11b+Gr1+ cells (representative data of 3 experiments) f) Serum cytokine levels 7 weeks post IL-17A MC injection compared to GFP MC control mice. Data pooled from two independent experiments and 14 mice. (Dotted line shows sensitivity of each assay. p<0.05 Mann-Whitney non-parametric test).