Table 3.
Relative gene expressions in hepatic tissues
| Groups | Normal | Alcohol only |
Alcohol with CGX treatment |
CGX 200 only | |
| 100 | 200 | ||||
| α-SMA | 1.00 | 1.26 ± 0.51a | 1.26 ± 0.42 | 0.97 ± 0.18c | 1.19 ± 0.18 |
| ColT1a1 | 1.00 | 1.72 ± 0.08b | 1.18 ± 0.14 | 1.01 ± 0.01d | 0.94 ± 0.53 |
| ColT1a2 | 1.00 | 1.63 ± 0.80 | 0.81 ± 0.10d | 0.74 ± 0.25d | 1.01 ± 0.41 |
| TIMP-1 | 1.00 | 1.64 ± 0.11b | 0.78 ± 0.04d | 0.79 ± 0.02d | 1.16 ± 0.06 |
| TIMP-2 | 1.00 | 1.72 ± 0.18b | 0.92 ± 0.03d | 0.92 ± 0.12d | 1.43 ± 0.14b |
| MMP-2 | 1.00 | 0.84 ± 0.63 | 1.23 ± 0.30c | 1.27 ± 0.17c | 1.27 ± 0.48 |
| MMP-9 | 1.00 | 0.72 ± 0.34 | 1.00 ± 0.81 | 1.10 ± 0.16c | 1.04 ± 0.78 |
| CYP2E1 | 1.00 | 1.33 ± 0.04b | 0.87 ± 0.03d | 0.84 ± 0.07d | 0.90 ± 0.14 |
| ALDH | 1.00 | 0.73 ± 0.18a | 0.81 ± 0.27 | 0.83 ± 0.13 | 1.13 ± 0.38 |
| TLR-4 | 1.00 | 1.44 ± 0.27a | 1.22 ± 0.08d | 0.95 ± 0.24c | 1.06 ± 0.04 |
| CCL 5 | 1.00 | 1.17 ± 0.07b | 1.06 ± 0.35 | 0.74 ± 0.31 | 1.08 ± 0.06 |
| MCP-1 | 1.00 | 1.48 ± 0.21b | 1.26 ± 0.10d | 1.50 ± 0.13 | 1.63 ± 0.09 |
Rats were orally administered with alcohol (10 mL of 30% ethanol/kg) with/without CGX (100 or 200 mg/kg) for 4 wk. At the end of the experiment, liver tissues were collected for mRNA expressions by real-time PCR. Data were expressed as mean of fold changes ± SD (n = 4)
P < 0.05,
P < 0.01 vs normal group,
P < 0.05,
P < 0.01 vs alcohol group. ALDH: Acetaldehydedehydrogenas; CCL 5: Chemokine (C-C motif) ligand-5; ColT1a1: Collagen type 1a1; ColT1a2: Collagen type 1a2; CYP2E1: cytochrome p450 2E1; MCP-1: Monocyte chemotactic protein-1; MMP-2: Matrix metalloproteinase-2; MMP-9: Matrix metalloproteinase-9; TIMP-1: Tissue inhibitor of metalloproteinases-1; TIMP-2: Tissue inhibitor of metalloproteinases-2; α-SMA: alpha-smooth muscle actin.